Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3458241..3458968 Replicon chromosome
Accession NZ_CP104788
Organism Escherichia coli strain SHP24

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N6H17_RS17030 Protein ID WP_000550189.1
Coordinates 3458654..3458968 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N6H17_RS17025 Protein ID WP_000560266.1
Coordinates 3458241..3458657 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H17_RS17015 (3453401) 3453401..3455752 + 2352 WP_000695487.1 alpha-glucosidase -
N6H17_RS17020 (3456178) 3456178..3458196 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N6H17_RS17025 (3458241) 3458241..3458657 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N6H17_RS17030 (3458654) 3458654..3458968 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N6H17_RS17035 (3459252) 3459252..3460388 - 1137 WP_187195868.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N6H17_RS17040 (3460473) 3460473..3460976 + 504 WP_001333820.1 M48 family metallopeptidase -
N6H17_RS17045 (3461053) 3461053..3461745 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
N6H17_RS17050 (3461824) 3461824..3462810 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T259172 WP_000550189.1 NZ_CP104788:c3458968-3458654 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT259172 WP_000560266.1 NZ_CP104788:c3458657-3458241 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References