Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 311582..312276 Replicon chromosome
Accession NZ_CP104788
Organism Escherichia coli strain SHP24

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N6H17_RS01455 Protein ID WP_001263489.1
Coordinates 311878..312276 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N6H17_RS01450 Protein ID WP_000554758.1
Coordinates 311582..311875 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H17_RS01430 (307214) 307214..307711 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N6H17_RS01435 (307935) 307935..309647 - 1713 Protein_279 flagellar biosynthesis protein FlhA -
N6H17_RS01440 (309619) 309619..310404 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N6H17_RS01445 (310475) 310475..311530 + 1056 WP_001226164.1 DNA polymerase IV -
N6H17_RS01450 (311582) 311582..311875 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N6H17_RS01455 (311878) 311878..312276 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N6H17_RS01460 (312286) 312286..312738 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
N6H17_RS01465 (313056) 313056..313262 + 207 Protein_285 RtcB family protein -
N6H17_RS01470 (313258) 313258..313779 + 522 Protein_286 peptide chain release factor H -
N6H17_RS01475 (313836) 313836..315293 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N6H17_RS01480 (315554) 315554..316012 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (316608) 316608..316688 + 81 NuclAT_11 - -
- (316608) 316608..316688 + 81 NuclAT_11 - -
- (316608) 316608..316688 + 81 NuclAT_11 - -
- (316608) 316608..316688 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T259158 WP_001263489.1 NZ_CP104788:311878-312276 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT259158 WP_000554758.1 NZ_CP104788:311582-311875 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References