Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3796656..3797197 Replicon chromosome
Accession NZ_CP104721
Organism Escherichia coli strain ECO3183

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag N5O92_RS18270 Protein ID WP_000615983.1
Coordinates 3796919..3797197 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag N5O92_RS18265 Protein ID WP_000729703.1
Coordinates 3796656..3796916 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O92_RS18245 (3792331) 3792331..3793116 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N5O92_RS18250 (3793088) 3793088..3794800 + 1713 Protein_3571 flagellar biosynthesis protein FlhA -
N5O92_RS18255 (3795024) 3795024..3795521 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N5O92_RS18260 (3795697) 3795697..3796446 - 750 WP_000056849.1 C40 family peptidase -
N5O92_RS18265 (3796656) 3796656..3796916 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5O92_RS18270 (3796919) 3796919..3797197 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5O92_RS18275 (3797353) 3797353..3798093 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5O92_RS18280 (3798064) 3798064..3798831 - 768 WP_000333380.1 class II glutamine amidotransferase -
N5O92_RS18285 (3799037) 3799037..3799615 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3790459..3805739 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T259050 WP_000615983.1 NZ_CP104721:3796919-3797197 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT259050 WP_000729703.1 NZ_CP104721:3796656-3796916 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References