Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3790459..3791153 Replicon chromosome
Accession NZ_CP104721
Organism Escherichia coli strain ECO3183

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N5O92_RS18230 Protein ID WP_001263489.1
Coordinates 3790459..3790857 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N5O92_RS18235 Protein ID WP_000554758.1
Coordinates 3790860..3791153 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3786047) 3786047..3786127 - 81 NuclAT_11 - -
- (3786047) 3786047..3786127 - 81 NuclAT_11 - -
- (3786047) 3786047..3786127 - 81 NuclAT_11 - -
- (3786047) 3786047..3786127 - 81 NuclAT_11 - -
N5O92_RS18205 (3786723) 3786723..3787181 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N5O92_RS18210 (3787442) 3787442..3788899 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N5O92_RS18215 (3788956) 3788956..3789477 - 522 Protein_3564 peptide chain release factor H -
N5O92_RS18220 (3789473) 3789473..3789679 - 207 Protein_3565 RtcB family protein -
N5O92_RS18225 (3789997) 3789997..3790449 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N5O92_RS18230 (3790459) 3790459..3790857 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5O92_RS18235 (3790860) 3790860..3791153 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5O92_RS18240 (3791205) 3791205..3792260 - 1056 WP_001226164.1 DNA polymerase IV -
N5O92_RS18245 (3792331) 3792331..3793116 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N5O92_RS18250 (3793088) 3793088..3794800 + 1713 Protein_3571 flagellar biosynthesis protein FlhA -
N5O92_RS18255 (3795024) 3795024..3795521 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3790459..3805739 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T259049 WP_001263489.1 NZ_CP104721:c3790857-3790459 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT259049 WP_000554758.1 NZ_CP104721:c3791153-3790860 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References