Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31723..32453 Replicon chromosome
Accession NZ_CP104689
Organism Enterobacter hormaechei strain 2020CK-00376

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag N5929_RS00150 Protein ID WP_023302825.1
Coordinates 31723..32037 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A837FJX0
Locus tag N5929_RS00155 Protein ID WP_015570115.1
Coordinates 32037..32453 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5929_RS00130 (N5929_13635) 27389..28474 + 1086 Protein_25 cellulase family glycosylhydrolase -
N5929_RS00135 (N5929_13630) 28380..29564 - 1185 WP_015570118.1 multidrug efflux MFS transporter EmrD -
N5929_RS00140 (N5929_13625) 29740..30573 - 834 WP_015570117.1 DMT family transporter -
N5929_RS00145 (N5929_13620) 30636..31082 - 447 WP_003861064.1 GNAT family N-acetyltransferase -
N5929_RS00150 (N5929_13610) 31723..32037 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
N5929_RS00155 (N5929_13605) 32037..32453 + 417 WP_015570115.1 helix-turn-helix domain-containing protein Antitoxin
N5929_RS00160 (N5929_13600) 32600..32698 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
N5929_RS00165 (N5929_13595) 32805..34493 + 1689 WP_032672176.1 acetolactate synthase large subunit -
N5929_RS00170 (N5929_13590) 34497..34784 + 288 WP_015570113.1 acetolactate synthase small subunit -
N5929_RS00175 (N5929_13585) 34910..35503 + 594 WP_003861054.1 transcriptional regulator UhpA -
N5929_RS00180 (N5929_13580) 35500..37005 + 1506 WP_047056921.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T259002 WP_023302825.1 NZ_CP104689:31723-32037 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15367.95 Da        Isoelectric Point: 5.7670

>AT259002 WP_015570115.1 NZ_CP104689:32037-32453 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FJX0

References