Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3849011..3849552 Replicon chromosome
Accession NZ_CP104681
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag N5922_RS19020 Protein ID WP_000615979.1
Coordinates 3849274..3849552 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N5922_RS19015 Protein ID WP_000729704.1
Coordinates 3849011..3849271 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5922_RS18995 (3844694) 3844694..3845479 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
N5922_RS19000 (3845451) 3845451..3847163 + 1713 Protein_3724 flagellar biosynthesis protein FlhA -
N5922_RS19005 (3847379) 3847379..3847876 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
N5922_RS19010 (3848052) 3848052..3848825 - 774 WP_001353765.1 C40 family peptidase -
N5922_RS19015 (3849011) 3849011..3849271 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5922_RS19020 (3849274) 3849274..3849552 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5922_RS19025 (3849708) 3849708..3850448 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5922_RS19030 (3850419) 3850419..3851186 - 768 WP_000333379.1 class II glutamine amidotransferase -
N5922_RS19035 (3851392) 3851392..3851970 - 579 WP_089584658.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T258996 WP_000615979.1 NZ_CP104681:3849274-3849552 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT258996 WP_000729704.1 NZ_CP104681:3849011-3849271 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References