Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3842822..3843516 Replicon chromosome
Accession NZ_CP104681
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag N5922_RS18980 Protein ID WP_001263493.1
Coordinates 3842822..3843220 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N5922_RS18985 Protein ID WP_000554757.1
Coordinates 3843223..3843516 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3838482) 3838482..3838562 - 81 NuclAT_10 - -
- (3838482) 3838482..3838562 - 81 NuclAT_10 - -
- (3838482) 3838482..3838562 - 81 NuclAT_10 - -
- (3838482) 3838482..3838562 - 81 NuclAT_10 - -
N5922_RS18950 (3837822) 3837822..3839066 - 1245 WP_000189541.1 esterase FrsA -
N5922_RS18955 (3839158) 3839158..3839616 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N5922_RS18960 (3839877) 3839877..3841334 + 1458 WP_001293002.1 cytosol nonspecific dipeptidase -
N5922_RS18965 (3841391) 3841391..3841912 - 522 Protein_3717 peptide chain release factor H -
N5922_RS18970 (3841911) 3841911..3842114 - 204 Protein_3718 RtcB family protein -
N5922_RS18975 (3842360) 3842360..3842812 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
N5922_RS18980 (3842822) 3842822..3843220 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5922_RS18985 (3843223) 3843223..3843516 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5922_RS18990 (3843568) 3843568..3844623 - 1056 WP_001226164.1 DNA polymerase IV -
N5922_RS18995 (3844694) 3844694..3845479 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
N5922_RS19000 (3845451) 3845451..3847163 + 1713 Protein_3724 flagellar biosynthesis protein FlhA -
N5922_RS19005 (3847379) 3847379..3847876 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T258995 WP_001263493.1 NZ_CP104681:c3843220-3842822 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258995 WP_000554757.1 NZ_CP104681:c3843516-3843223 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References