Detailed information of TA system TA258980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 706806..707533
• Replicon: chromosome
• Accession: NZ_CP104681
• Organism: Escherichia coli strain 2022CK-00450

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: N5922_RS03540
• Protein ID: WP_000550192.1
• Coordinates: 706806..707120 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N5922_RS03545
• Protein ID: WP_000560266.1
• Coordinates: 707117..707533 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5922_RS03520 (702973)	702973..703959	-	987	WP_001301393.1	Gfo/Idh/MocA family oxidoreductase	-
N5922_RS03525 (704038)	704038..704721	-	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
N5922_RS03530 (704798)	704798..705301	-	504	WP_001295542.1	M48 family metallopeptidase	-
N5922_RS03535 (705386)	705386..706522	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
N5922_RS03540 (706806)	706806..707120	+	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
N5922_RS03545 (707117)	707117..707533	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
N5922_RS03550 (707578)	707578..709596	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
N5922_RS03555 (710022)	710022..712373	-	2352	WP_261588598.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T258980 WP_000550192.1 NZ_CP104681:706806-707120 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T258980 NZ_CP104681:706806-707120 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT258980 WP_000560266.1 NZ_CP104681:707117-707533 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT258980 NZ_CP104681:707117-707533 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1EX98