Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 657007..657806 Replicon chromosome
Accession NZ_CP104681
Organism Escherichia coli strain 2022CK-00450

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag N5922_RS03290 Protein ID WP_000347251.1
Coordinates 657007..657471 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N5922_RS03295 Protein ID WP_001307405.1
Coordinates 657471..657806 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5922_RS03260 (652008) 652008..652442 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N5922_RS03265 (652460) 652460..653338 - 879 WP_222214802.1 PTS N-acetylgalactosamine transporter subunit IID -
N5922_RS03270 (653328) 653328..654107 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5922_RS03275 (654118) 654118..654591 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5922_RS03280 (654614) 654614..655894 - 1281 WP_000681905.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5922_RS03285 (656143) 656143..656952 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N5922_RS03290 (657007) 657007..657471 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5922_RS03295 (657471) 657471..657806 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5922_RS03300 (657955) 657955..659526 - 1572 WP_001273753.1 galactarate dehydratase -
N5922_RS03305 (659901) 659901..661235 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N5922_RS03310 (661251) 661251..662021 + 771 WP_089584629.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T258979 WP_000347251.1 NZ_CP104681:c657471-657007 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT258979 WP_001307405.1 NZ_CP104681:c657806-657471 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References