Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3964337..3964934 Replicon chromosome
Accession NZ_CP104678
Organism Klebsiella pneumoniae strain 2021CK-00608

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag N5933_RS19515 Protein ID WP_004142563.1
Coordinates 3964617..3964934 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag N5933_RS19510 Protein ID WP_004142561.1
Coordinates 3964337..3964624 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5933_RS19480 (3960417) 3960417..3960665 + 249 WP_020325243.1 DUF1158 domain-containing protein -
N5933_RS19485 (3960683) 3960683..3961024 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
N5933_RS19490 (3961055) 3961055..3962170 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
N5933_RS19495 (3962350) 3962350..3962931 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
N5933_RS19500 (3962931) 3962931..3963299 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
N5933_RS19505 (3963419) 3963419..3964072 + 654 WP_117040415.1 oxygen-insensitive NAD(P)H nitroreductase -
N5933_RS19510 (3964337) 3964337..3964624 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
N5933_RS19515 (3964617) 3964617..3964934 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5933_RS19520 (3965119) 3965119..3966162 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
N5933_RS19525 (3966832) 3966832..3967698 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
N5933_RS19530 (3967807) 3967807..3969234 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T258968 WP_004142563.1 NZ_CP104678:c3964934-3964617 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT258968 WP_004142561.1 NZ_CP104678:c3964624-3964337 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References