Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4909759..4910361 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N5914_RS23605 Protein ID WP_000897305.1
Coordinates 4910050..4910361 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5914_RS23600 Protein ID WP_000356397.1
Coordinates 4909759..4910049 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS23580 (4906261) 4906261..4907163 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N5914_RS23585 (4907160) 4907160..4907795 + 636 WP_039052566.1 formate dehydrogenase cytochrome b556 subunit -
N5914_RS23590 (4907792) 4907792..4908721 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N5914_RS23595 (4908936) 4908936..4909154 - 219 WP_001298592.1 CopG family transcriptional regulator -
N5914_RS23600 (4909759) 4909759..4910049 - 291 WP_000356397.1 NadS family protein Antitoxin
N5914_RS23605 (4910050) 4910050..4910361 - 312 WP_000897305.1 hypothetical protein Toxin
N5914_RS23610 (4910590) 4910590..4911498 + 909 WP_001318161.1 alpha/beta hydrolase -
N5914_RS23615 (4911562) 4911562..4912503 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N5914_RS23620 (4912548) 4912548..4912985 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N5914_RS23625 (4912982) 4912982..4913854 - 873 WP_000920762.1 virulence factor BrkB family protein -
N5914_RS23630 (4913848) 4913848..4914447 - 600 WP_001314324.1 glucose-1-phosphatase -
N5914_RS23635 (4914546) 4914546..4915331 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258935 WP_000897305.1 NZ_CP104665:c4910361-4910050 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258935 WP_000356397.1 NZ_CP104665:c4910049-4909759 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References