258926

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3972571..3973265 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag N5914_RS19175 Protein ID WP_001263491.1
Coordinates 3972571..3972969 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag N5914_RS19180 Protein ID WP_000554755.1
Coordinates 3972972..3973265 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS19145 (3967833) 3967833..3969290 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
N5914_RS19150 (3969299) 3969299..3969580 + 282 WP_022645226.1 hypothetical protein -
N5914_RS19155 (3969597) 3969597..3970106 - 510 WP_001361775.1 metal-dependent hydrolase -
N5914_RS19160 (3970168) 3970168..3970782 - 615 WP_022645225.1 peptide chain release factor H -
N5914_RS19165 (3970779) 3970779..3971918 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
N5914_RS19170 (3972109) 3972109..3972561 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
N5914_RS19175 (3972571) 3972571..3972969 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5914_RS19180 (3972972) 3972972..3973265 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5914_RS19185 (3973317) 3973317..3974372 - 1056 WP_022645222.1 DNA polymerase IV -
N5914_RS19190 (3974443) 3974443..3975228 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
N5914_RS19195 (3975200) 3975200..3976912 + 1713 Protein_3765 flagellar biosynthesis protein FlhA -
N5914_RS19200 (3977010) 3977010..3977783 - 774 WP_022645219.1 C40 family peptidase -
N5914_RS19205 (3977969) 3977969..3978229 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T258926 WP_001263491.1 NZ_CP104665:c3972969-3972571 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT258926 WP_000554755.1 NZ_CP104665:c3973265-3972972 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References