Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 630191..630990 Replicon chromosome
Accession NZ_CP104665
Organism Escherichia coli strain 2021CK-00607

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag N5914_RS03000 Protein ID WP_000347273.1
Coordinates 630191..630655 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag N5914_RS03005 Protein ID WP_001308975.1
Coordinates 630655..630990 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5914_RS02970 (625192) 625192..625626 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N5914_RS02975 (625644) 625644..626522 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
N5914_RS02980 (626512) 626512..627291 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5914_RS02985 (627302) 627302..627775 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5914_RS02990 (627798) 627798..629078 - 1281 WP_023908831.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5914_RS02995 (629327) 629327..630136 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N5914_RS03000 (630191) 630191..630655 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5914_RS03005 (630655) 630655..630990 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5914_RS03010 (631139) 631139..632710 - 1572 WP_023908830.1 galactarate dehydratase -
N5914_RS03015 (633085) 633085..634419 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
N5914_RS03020 (634435) 634435..635205 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T258913 WP_000347273.1 NZ_CP104665:c630655-630191 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT258913 WP_001308975.1 NZ_CP104665:c630990-630655 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References