Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 328618..329204 Replicon chromosome
Accession NZ_CP104660
Organism Klebsiella pneumoniae strain 2021CK-01729

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag N4T33_RS01525 Protein ID WP_002920800.1
Coordinates 328836..329204 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag N4T33_RS01520 Protein ID WP_004174006.1
Coordinates 328618..328839 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T33_RS01500 (N4T33_01500) 324775..325701 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
N4T33_RS01505 (N4T33_01505) 325698..326975 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
N4T33_RS01510 (N4T33_01510) 326972..327739 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
N4T33_RS01515 (N4T33_01515) 327741..328454 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
N4T33_RS01520 (N4T33_01520) 328618..328839 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
N4T33_RS01525 (N4T33_01525) 328836..329204 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
N4T33_RS01530 (N4T33_01530) 329477..330793 + 1317 WP_019704662.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
N4T33_RS01535 (N4T33_01535) 330900..331787 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
N4T33_RS01540 (N4T33_01540) 331784..332629 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
N4T33_RS01545 (N4T33_01545) 332631..333701 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 325698..334438 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T258896 WP_002920800.1 NZ_CP104660:328836-329204 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT258896 WP_004174006.1 NZ_CP104660:328618-328839 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References