Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3604882..3605528 Replicon chromosome
Accession NZ_CP104659
Organism Klebsiella pneumoniae strain 2020CK-00096

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag N5936_RS17720 Protein ID WP_016529833.1
Coordinates 3605181..3605528 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag N5936_RS17715 Protein ID WP_002920557.1
Coordinates 3604882..3605181 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5936_RS17695 (3601088) 3601088..3601846 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
N5936_RS17700 (3601896) 3601896..3602726 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
N5936_RS17705 (3602777) 3602777..3603106 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
N5936_RS17710 (3603311) 3603311..3604819 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
N5936_RS17715 (3604882) 3604882..3605181 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
N5936_RS17720 (3605181) 3605181..3605528 - 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5936_RS17725 (3605694) 3605694..3608141 - 2448 WP_002920561.1 glycogen phosphorylase -
N5936_RS17730 (3608159) 3608159..3609592 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T258888 WP_016529833.1 NZ_CP104659:c3605528-3605181 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT258888 WP_002920557.1 NZ_CP104659:c3605181-3604882 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References