Detailed information of TA system TA258880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 96935..97520
• Replicon: plasmid unnamed2
• Accession: NZ_CP104653
• Organism: Acinetobacter variabilis strain 2021CK-01005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: N5980_RS16870
• Protein ID: WP_000897307.1
• Coordinates: 96935..97255 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N5980_RS16875
• Protein ID: WP_000369781.1
• Coordinates: 97248..97520 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5980_RS16840 (N5980_16825)	92208..92738	-	531	WP_000172759.1	hypothetical protein	-
N5980_RS16845 (N5980_16830)	92887..93189	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N5980_RS16850 (N5980_16835)	93190..93471	+	282	WP_000985609.1	putative addiction module antidote protein	-
N5980_RS16855 (N5980_16840)	93571..94779	+	1209	WP_042090285.1	IS256 family transposase	-
N5980_RS16860 (N5980_16845)	94890..96215	-	1326	WP_234622163.1	ISNCY family transposase	-
N5980_RS16865 (N5980_16850)	96376..96795	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
N5980_RS16870 (N5980_16855)	96935..97255	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N5980_RS16875 (N5980_16860)	97248..97520	+	273	WP_000369781.1	NadS family protein	Antitoxin
N5980_RS16880 (N5980_16865)	98013..99488	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
N5980_RS16885 (N5980_16870)	99544..100428	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
N5980_RS16890 (N5980_16875)	100681..102006	+	1326	WP_234622163.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / blaNDM-1 / aph(3')-VIa / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / aac(3)-IId	-	1..123124	123124
	-	inside	IScluster/Tn	aph(3')-VIa / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / aac(3)-IId	-	86409..116064	29655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T258880 WP_000897307.1 NZ_CP104653:96935-97255 [Acinetobacter variabilis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T258880 NZ_CP104653:96935-97255 [Acinetobacter variabilis]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT258880 WP_000369781.1 NZ_CP104653:97248-97520 [Acinetobacter variabilis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT258880 NZ_CP104653:97248-97520 [Acinetobacter variabilis]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3