Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4570312..4571006 Replicon chromosome
Accession NZ_CP104649
Organism Escherichia coli strain PNUSAE008512

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag N5913_RS23115 Protein ID WP_001263495.1
Coordinates 4570608..4571006 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N5913_RS23110 Protein ID WP_000554757.1
Coordinates 4570312..4570605 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5913_RS23090 (4566023) 4566023..4566520 + 498 Protein_4503 REP-associated tyrosine transposase RayT -
N5913_RS23095 (4566665) 4566665..4568377 - 1713 Protein_4504 flagellar biosynthesis protein FlhA -
N5913_RS23100 (4568349) 4568349..4569134 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
N5913_RS23105 (4569205) 4569205..4570260 + 1056 WP_001226186.1 DNA polymerase IV -
N5913_RS23110 (4570312) 4570312..4570605 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5913_RS23115 (4570608) 4570608..4571006 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5913_RS23120 (4571016) 4571016..4571468 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
N5913_RS23125 (4571787) 4571787..4571990 + 204 Protein_4510 RtcB family protein -
N5913_RS23130 (4571989) 4571989..4572510 + 522 Protein_4511 peptide chain release factor H -
N5913_RS23135 (4572567) 4572567..4574024 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
N5913_RS23140 (4574285) 4574285..4574743 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4575339) 4575339..4575419 + 81 NuclAT_11 - -
- (4575339) 4575339..4575419 + 81 NuclAT_11 - -
- (4575339) 4575339..4575419 + 81 NuclAT_11 - -
- (4575339) 4575339..4575419 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T258869 WP_001263495.1 NZ_CP104649:4570608-4571006 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258869 WP_000554757.1 NZ_CP104649:4570312-4570605 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References