Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3226352..3227046 Replicon chromosome
Accession NZ_CP104645
Organism Escherichia coli strain PNUSAE019785

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag N5932_RS15885 Protein ID WP_001263495.1
Coordinates 3226648..3227046 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N5932_RS15880 Protein ID WP_000554757.1
Coordinates 3226352..3226645 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5932_RS15860 (3222063) 3222063..3222560 + 498 Protein_3075 REP-associated tyrosine transposase RayT -
N5932_RS15865 (3222705) 3222705..3224417 - 1713 Protein_3076 flagellar biosynthesis protein FlhA -
N5932_RS15870 (3224389) 3224389..3225174 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
N5932_RS15875 (3225245) 3225245..3226300 + 1056 WP_001226186.1 DNA polymerase IV -
N5932_RS15880 (3226352) 3226352..3226645 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5932_RS15885 (3226648) 3226648..3227046 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5932_RS15890 (3227056) 3227056..3227508 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
N5932_RS15895 (3227827) 3227827..3228030 + 204 Protein_3082 RtcB family protein -
N5932_RS15900 (3228029) 3228029..3228550 + 522 Protein_3083 peptide chain release factor H -
N5932_RS15905 (3228607) 3228607..3230064 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
N5932_RS15910 (3230325) 3230325..3230783 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3231379) 3231379..3231459 + 81 NuclAT_11 - -
- (3231379) 3231379..3231459 + 81 NuclAT_11 - -
- (3231379) 3231379..3231459 + 81 NuclAT_11 - -
- (3231379) 3231379..3231459 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T258796 WP_001263495.1 NZ_CP104645:3226648-3227046 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258796 WP_000554757.1 NZ_CP104645:3226352-3226645 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References