Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3078422..3079116 Replicon chromosome
Accession NZ_CP104639
Organism Escherichia coli strain PNUSAE014970

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag N4555_RS14760 Protein ID WP_001263495.1
Coordinates 3078718..3079116 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N4555_RS14755 Protein ID WP_000554757.1
Coordinates 3078422..3078715 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4555_RS14735 (3074133) 3074133..3074630 + 498 Protein_2859 REP-associated tyrosine transposase RayT -
N4555_RS14740 (3074775) 3074775..3076487 - 1713 Protein_2860 flagellar biosynthesis protein FlhA -
N4555_RS14745 (3076459) 3076459..3077244 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
N4555_RS14750 (3077315) 3077315..3078370 + 1056 WP_001226188.1 DNA polymerase IV -
N4555_RS14755 (3078422) 3078422..3078715 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N4555_RS14760 (3078718) 3078718..3079116 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N4555_RS14765 (3079126) 3079126..3079578 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
N4555_RS14770 (3079897) 3079897..3080100 + 204 Protein_2866 RtcB family protein -
N4555_RS14775 (3080099) 3080099..3080620 + 522 Protein_2867 peptide chain release factor H -
N4555_RS14780 (3080677) 3080677..3082134 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
N4555_RS14785 (3082395) 3082395..3082853 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3083449) 3083449..3083529 + 81 NuclAT_12 - -
- (3083449) 3083449..3083529 + 81 NuclAT_12 - -
- (3083449) 3083449..3083529 + 81 NuclAT_12 - -
- (3083449) 3083449..3083529 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T258722 WP_001263495.1 NZ_CP104639:3078718-3079116 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258722 WP_000554757.1 NZ_CP104639:3078422-3078715 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References