Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 13343..13884 Replicon plasmid pHNAH212872-2
Accession NZ_CP104632
Organism Escherichia coli strain AHM21C2872I

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag N5855_RS25610 Protein ID WP_000615980.1
Coordinates 13343..13621 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag N5855_RS25615 Protein ID WP_000729709.1
Coordinates 13624..13884 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5855_RS25595 (10925) 10925..11503 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N5855_RS25600 (11709) 11709..12476 + 768 WP_000333379.1 class II glutamine amidotransferase -
N5855_RS25605 (12447) 12447..13187 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5855_RS25610 (13343) 13343..13621 - 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5855_RS25615 (13624) 13624..13884 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5855_RS25620 (14094) 14094..14843 + 750 WP_000056850.1 C40 family peptidase -
N5855_RS25625 (15019) 15019..15516 + 498 WP_021556447.1 REP-associated tyrosine transposase RayT -
N5855_RS25630 (15709) 15709..17802 - 2094 WP_021556448.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - vgrG/tssI / rhs/PAAR / gmhA/lpcA 1..100861 100861


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T258692 WP_000615980.1 NZ_CP104632:c13621-13343 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT258692 WP_000729709.1 NZ_CP104632:c13884-13624 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References