258685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4357699..4358196 Replicon chromosome
Accession NZ_CP104629
Organism Escherichia coli strain AHM21C2872I

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag N5855_RS20860 Protein ID WP_001672275.1
Coordinates 4358023..4358196 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N5855_RS20855 Protein ID WP_000398619.1
Coordinates 4357699..4357995 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5855_RS20835 (4354430) 4354430..4355149 - 720 WP_000611288.1 two-component system response regulator DcuR -
N5855_RS20840 (4355146) 4355146..4356777 - 1632 WP_001216477.1 sensor histidine kinase -
N5855_RS20845 (4356958) 4356958..4357188 + 231 WP_021557455.1 4Fe-4S mono-cluster protein YjdI -
N5855_RS20850 (4357200) 4357200..4357472 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5855_RS20855 (4357699) 4357699..4357995 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5855_RS20860 (4358023) 4358023..4358196 + 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
N5855_RS20865 (4358315) 4358315..4359832 - 1518 WP_001295074.1 lysine--tRNA ligase -
N5855_RS20870 (4360069) 4360069..4361526 - 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4357699..4367050 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T258685 WP_001672275.1 NZ_CP104629:4358023-4358196 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT258685 WP_000398619.1 NZ_CP104629:4357699-4357995 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References