Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4033032..4033634 Replicon chromosome
Accession NZ_CP104629
Organism Escherichia coli strain AHM21C2872I

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N5855_RS19320 Protein ID WP_000897305.1
Coordinates 4033032..4033343 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5855_RS19325 Protein ID WP_000356397.1
Coordinates 4033344..4033634 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5855_RS19290 (4028062) 4028062..4028847 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
N5855_RS19295 (4028946) 4028946..4029545 + 600 WP_001295269.1 glucose-1-phosphatase -
N5855_RS19300 (4029539) 4029539..4030411 + 873 WP_000920762.1 virulence factor BrkB family protein -
N5855_RS19305 (4030408) 4030408..4030845 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N5855_RS19310 (4030890) 4030890..4031831 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N5855_RS19315 (4031895) 4031895..4032803 - 909 WP_001162704.1 alpha/beta hydrolase -
N5855_RS19320 (4033032) 4033032..4033343 + 312 WP_000897305.1 hypothetical protein Toxin
N5855_RS19325 (4033344) 4033344..4033634 + 291 WP_000356397.1 NadS family protein Antitoxin
N5855_RS19330 (4034220) 4034220..4034438 + 219 WP_001315930.1 CopG family transcriptional regulator -
N5855_RS19335 (4034658) 4034658..4034900 + 243 WP_001087409.1 protein YiiF -
N5855_RS19340 (4035230) 4035230..4036159 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
N5855_RS19345 (4036156) 4036156..4036791 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N5855_RS19350 (4036788) 4036788..4037690 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258684 WP_000897305.1 NZ_CP104629:4033032-4033343 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258684 WP_000356397.1 NZ_CP104629:4033344-4033634 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References