Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3820379..3820976 Replicon chromosome
Accession NZ_CP104627
Organism Klebsiella pneumoniae strain AHM21C2836KI

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag N5857_RS18640 Protein ID WP_004142563.1
Coordinates 3820659..3820976 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag N5857_RS18635 Protein ID WP_004142561.1
Coordinates 3820379..3820666 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5857_RS18605 (3816459) 3816459..3816707 + 249 WP_002893037.1 DUF1158 domain-containing protein -
N5857_RS18610 (3816725) 3816725..3817066 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
N5857_RS18615 (3817097) 3817097..3818212 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
N5857_RS18620 (3818392) 3818392..3818973 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
N5857_RS18625 (3818973) 3818973..3819341 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
N5857_RS18630 (3819461) 3819461..3820114 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
N5857_RS18635 (3820379) 3820379..3820666 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
N5857_RS18640 (3820659) 3820659..3820976 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5857_RS18645 (3821161) 3821161..3822204 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
N5857_RS18650 (3822870) 3822870..3823736 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
N5857_RS18655 (3823845) 3823845..3825272 + 1428 WP_040181878.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T258664 WP_004142563.1 NZ_CP104627:c3820976-3820659 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT258664 WP_004142561.1 NZ_CP104627:c3820666-3820379 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References