Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3628423..3628964 Replicon chromosome
Accession NZ_CP104618
Organism Escherichia coli strain AHM9C68I

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag N5859_RS17745 Protein ID WP_000615976.1
Coordinates 3628686..3628964 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N5859_RS17740 Protein ID WP_000729704.1
Coordinates 3628423..3628683 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5859_RS17720 (3624098) 3624098..3624883 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
N5859_RS17725 (3624855) 3624855..3626567 + 1713 Protein_3468 flagellar biosynthesis protein FlhA -
N5859_RS17730 (3626791) 3626791..3627288 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N5859_RS17735 (3627464) 3627464..3628237 - 774 WP_015675129.1 C40 family peptidase -
N5859_RS17740 (3628423) 3628423..3628683 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5859_RS17745 (3628686) 3628686..3628964 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5859_RS17750 (3629120) 3629120..3629860 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5859_RS17755 (3629831) 3629831..3630598 - 768 WP_000333379.1 class II glutamine amidotransferase -
N5859_RS17760 (3630804) 3630804..3631382 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3621264..3644481 23217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T258639 WP_000615976.1 NZ_CP104618:3628686-3628964 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT258639 WP_000729704.1 NZ_CP104618:3628423-3628683 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References