Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4043063..4043862 Replicon chromosome
Accession NZ_CP104613
Organism Escherichia coli strain AHM8C212RI

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag N5856_RS19455 Protein ID WP_000347273.1
Coordinates 4043398..4043862 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N5856_RS19450 Protein ID WP_001307405.1
Coordinates 4043063..4043398 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5856_RS19435 (4038847) 4038847..4039617 - 771 WP_024250085.1 2-dehydro-3-deoxyglucarate aldolase -
N5856_RS19440 (4039633) 4039633..4040967 - 1335 WP_097702292.1 galactarate/glucarate/glycerate transporter GarP -
N5856_RS19445 (4041342) 4041342..4042913 + 1572 WP_097489939.1 galactarate dehydratase -
N5856_RS19450 (4043063) 4043063..4043398 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5856_RS19455 (4043398) 4043398..4043862 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5856_RS19460 (4043917) 4043917..4044726 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
N5856_RS19465 (4044975) 4044975..4046255 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5856_RS19470 (4046278) 4046278..4046751 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5856_RS19475 (4046762) 4046762..4047541 + 780 WP_033557704.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5856_RS19480 (4047531) 4047531..4048409 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
N5856_RS19485 (4048427) 4048427..4048861 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4033799..4043862 10063


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T258616 WP_000347273.1 NZ_CP104613:4043398-4043862 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT258616 WP_001307405.1 NZ_CP104613:4043063-4043398 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References