Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 153703..154430 Replicon plasmid pHNAH8161B-2
Accession NZ_CP104605
Organism Klebsiella pneumoniae strain AHM8C161BI

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag N5862_RS28580 Protein ID WP_011251285.1
Coordinates 154119..154430 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5862_RS28575 Protein ID WP_011251286.1
Coordinates 153703..154122 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5862_RS28550 (N5862_28540) 149295..150662 + 1368 WP_017900870.1 formimidoylglutamate deiminase -
N5862_RS28555 (N5862_28545) 151187..151722 + 536 Protein_168 transposase -
N5862_RS28560 (N5862_28550) 151836..152102 - 267 WP_017900868.1 hypothetical protein -
N5862_RS28565 (N5862_28555) 152206..153174 + 969 Protein_170 IS5 family transposase -
N5862_RS28570 (N5862_28560) 153220..153633 - 414 WP_017901337.1 hypothetical protein -
N5862_RS28575 (N5862_28565) 153703..154122 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
N5862_RS28580 (N5862_28570) 154119..154430 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
N5862_RS28585 (N5862_28575) 154635..155072 - 438 Protein_174 DDE-type integrase/transposase/recombinase -
N5862_RS28590 (N5862_28580) 155186..155563 + 378 WP_004118218.1 transposase -
N5862_RS28595 (N5862_28585) 155560..155907 + 348 WP_032430752.1 IS66 family insertion sequence element accessory protein TnpB -
N5862_RS28600 (N5862_28590) 155956..157494 + 1539 WP_017901237.1 IS66 family transposase -
N5862_RS28605 (N5862_28595) 157534..157713 + 180 Protein_178 IS5/IS1182 family transposase -
N5862_RS28610 (N5862_28600) 158259..159239 - 981 WP_012569499.1 IS5-like element ISKpn26 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB4 / blaDHA-1 / sul1 / blaTEM-1B / mph(A) / qacE / aadA2 / dfrA12 - 1..218818 218818
- inside IScluster/Tn blaTEM-1B - 151504..164103 12599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T258591 WP_011251285.1 NZ_CP104605:c154430-154119 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT258591 WP_011251286.1 NZ_CP104605:c154122-153703 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References