Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2127452..2128014 Replicon chromosome
Accession NZ_CP104598
Organism Escherichia coli strain AHM8C160RI

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag N5861_RS10190 Protein ID WP_000605676.1
Coordinates 2127736..2128014 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag N5861_RS10185 Protein ID WP_000781370.1
Coordinates 2127452..2127736 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5861_RS10170 (2124255) 2124255..2125139 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
N5861_RS10175 (2125132) 2125132..2125785 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
N5861_RS10180 (2126019) 2126019..2127347 - 1329 WP_024250060.1 IS4-like element IS4 family transposase -
N5861_RS10185 (2127452) 2127452..2127736 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
N5861_RS10190 (2127736) 2127736..2128014 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5861_RS10195 (2128200) 2128200..2129210 - 1011 WP_250308366.1 alcohol dehydrogenase AdhP -
N5861_RS10200 (2129344) 2129344..2131041 - 1698 WP_000433465.1 malate dehydrogenase -
N5861_RS10205 (2131198) 2131198..2131335 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
N5861_RS10210 (2131437) 2131437..2131652 - 216 WP_000495766.1 biofilm-dependent modulation protein -
N5861_RS10215 (2131998) 2131998..2132429 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2126019..2127347 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T258552 WP_000605676.1 NZ_CP104598:c2128014-2127736 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT258552 WP_000781370.1 NZ_CP104598:c2127736-2127452 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References