Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4722258..4722860 Replicon chromosome
Accession NZ_CP104595
Organism Escherichia coli strain EC9952

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N5B57_RS22820 Protein ID WP_000897305.1
Coordinates 4722549..4722860 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5B57_RS22815 Protein ID WP_000356397.1
Coordinates 4722258..4722548 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5B57_RS22790 (4718183) 4718183..4719085 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N5B57_RS22795 (4719082) 4719082..4719717 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N5B57_RS22800 (4719714) 4719714..4720643 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
N5B57_RS22805 (4720973) 4720973..4721215 - 243 WP_001087409.1 protein YiiF -
N5B57_RS22810 (4721435) 4721435..4721653 - 219 WP_001315930.1 CopG family transcriptional regulator -
N5B57_RS22815 (4722258) 4722258..4722548 - 291 WP_000356397.1 NadS family protein Antitoxin
N5B57_RS22820 (4722549) 4722549..4722860 - 312 WP_000897305.1 hypothetical protein Toxin
N5B57_RS22825 (4723089) 4723089..4723997 + 909 WP_021293166.1 alpha/beta hydrolase -
N5B57_RS22830 (4724061) 4724061..4725002 - 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
N5B57_RS22835 (4725047) 4725047..4725484 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N5B57_RS22840 (4725481) 4725481..4726353 - 873 WP_000920762.1 virulence factor BrkB family protein -
N5B57_RS22845 (4726347) 4726347..4726946 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258538 WP_000897305.1 NZ_CP104595:c4722860-4722549 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258538 WP_000356397.1 NZ_CP104595:c4722548-4722258 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References