258368

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604995..605794 Replicon chromosome
Accession NZ_CP104539
Organism Escherichia coli strain E6

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag N5O76_RS02985 Protein ID WP_000347267.1
Coordinates 604995..605459 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N5O76_RS02990 Protein ID WP_001307405.1
Coordinates 605459..605794 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O76_RS02955 (599996) 599996..600430 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N5O76_RS02960 (600448) 600448..601326 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N5O76_RS02965 (601316) 601316..602095 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5O76_RS02970 (602106) 602106..602579 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5O76_RS02975 (602602) 602602..603882 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5O76_RS02980 (604131) 604131..604940 + 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
N5O76_RS02985 (604995) 604995..605459 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5O76_RS02990 (605459) 605459..605794 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5O76_RS02995 (605943) 605943..607514 - 1572 WP_001273741.1 galactarate dehydratase -
N5O76_RS03000 (607889) 607889..609223 + 1335 WP_160348001.1 galactarate/glucarate/glycerate transporter GarP -
N5O76_RS03005 (609239) 609239..610009 + 771 WP_261306653.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 604995..617729 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T258368 WP_000347267.1 NZ_CP104539:c605459-604995 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT258368 WP_001307405.1 NZ_CP104539:c605794-605459 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References