Detailed information of TA system TA258364

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79604..80030
• Replicon: plasmid p1
• Accession: NZ_CP104537
• Organism: Escherichia coli strain E3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: N5O72_RS23135
• Protein ID: WP_001372321.1
• Coordinates: 79604..79729 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79806..80030 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N5O72_RS23095 (74973)	74973..75662	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
N5O72_RS23100 (75849)	75849..76232	-	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
N5O72_RS23105 (76553)	76553..77155	+	603	WP_224476533.1	transglycosylase SLT domain-containing protein	-
N5O72_RS23110 (77452)	77452..78273	-	822	WP_001234469.1	DUF932 domain-containing protein	-
N5O72_RS23115 (78395)	78395..78682	-	288	WP_000107535.1	hypothetical protein	-
N5O72_RS23120 (78707)	78707..78913	-	207	WP_000547939.1	hypothetical protein	-
N5O72_RS23125 (78983)	78983..79156	+	174	Protein_83	hypothetical protein	-
N5O72_RS23130 (79154)	79154..79384	-	231	WP_071587244.1	hypothetical protein	-
N5O72_RS23135 (79604)	79604..79729	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
N5O72_RS23140 (79671)	79671..79820	-	150	Protein_86	plasmid maintenance protein Mok	-
- (79806)	79806..80030	-	225	NuclAT_0	-	Antitoxin
- (79806)	79806..80030	-	225	NuclAT_0	-	Antitoxin
- (79806)	79806..80030	-	225	NuclAT_0	-	Antitoxin
- (79806)	79806..80030	-	225	NuclAT_0	-	Antitoxin
N5O72_RS23145 (79842)	79842..80030	+	189	WP_001299721.1	hypothetical protein	-
N5O72_RS23150 (79999)	79999..80761	-	763	Protein_88	plasmid SOS inhibition protein A	-
N5O72_RS23155 (80758)	80758..81192	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
N5O72_RS23160 (81247)	81247..83205	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
N5O72_RS23165 (83271)	83271..83504	-	234	WP_000005990.1	DUF905 family protein	-
N5O72_RS23170 (83567)	83567..84064	-	498	WP_089552049.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T258364 WP_001372321.1 NZ_CP104537:c79729-79604 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT258364 NZ_CP104537:c80030-79806 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin