Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4188277..4188774 Replicon chromosome
Accession NZ_CP104536
Organism Escherichia coli strain E3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N5O72_RS20315 Protein ID WP_001173343.1
Coordinates 4188277..4188450 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag N5O72_RS20320 Protein ID WP_001425728.1
Coordinates 4188478..4188774 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O72_RS20305 (4184947) 4184947..4186404 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
N5O72_RS20310 (4186641) 4186641..4188158 + 1518 WP_001295074.1 lysine--tRNA ligase -
N5O72_RS20315 (4188277) 4188277..4188450 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N5O72_RS20320 (4188478) 4188478..4188774 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N5O72_RS20325 (4189001) 4189001..4189273 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N5O72_RS20330 (4189285) 4189285..4189515 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
N5O72_RS20335 (4189696) 4189696..4191327 + 1632 WP_001216487.1 sensor histidine kinase -
N5O72_RS20340 (4191324) 4191324..4192043 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4179423..4188774 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T258363 WP_001173343.1 NZ_CP104536:c4188450-4188277 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT258363 WP_001425728.1 NZ_CP104536:c4188774-4188478 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References