Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3856890..3857584 Replicon chromosome
Accession NZ_CP104533
Organism Escherichia coli strain E10

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag N5O77_RS19220 Protein ID WP_001263500.1
Coordinates 3856890..3857288 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N5O77_RS19225 Protein ID WP_000554758.1
Coordinates 3857291..3857584 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O77_RS19195 (3852255) 3852255..3852713 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
N5O77_RS19200 (3852974) 3852974..3854431 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
N5O77_RS19205 (3854488) 3854488..3855102 - 615 WP_000602123.1 peptide chain release factor H -
N5O77_RS19210 (3855099) 3855099..3856238 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
N5O77_RS19215 (3856428) 3856428..3856880 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
N5O77_RS19220 (3856890) 3856890..3857288 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5O77_RS19225 (3857291) 3857291..3857584 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5O77_RS19230 (3857636) 3857636..3858691 - 1056 WP_001226168.1 DNA polymerase IV -
N5O77_RS19235 (3858762) 3858762..3859547 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
N5O77_RS19240 (3859519) 3859519..3861231 + 1713 Protein_3770 flagellar biosynthesis protein FlhA -
N5O77_RS19245 (3861310) 3861310..3861468 + 159 WP_014639450.1 hypothetical protein -
N5O77_RS19250 (3861551) 3861551..3862048 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3856890..3877244 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T258331 WP_001263500.1 NZ_CP104533:c3857288-3856890 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT258331 WP_000554758.1 NZ_CP104533:c3857584-3857291 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References