Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3627970..3628649 Replicon chromosome
Accession NZ_CP104533
Organism Escherichia coli strain E10

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag N5O77_RS18165 Protein ID WP_261175519.1
Coordinates 3628347..3628649 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N5O77_RS18160 Protein ID WP_000806442.1
Coordinates 3627970..3628311 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O77_RS18150 (3624214) 3624214..3625146 - 933 WP_000883052.1 glutaminase A -
N5O77_RS18155 (3625408) 3625408..3627912 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
N5O77_RS18160 (3627970) 3627970..3628311 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N5O77_RS18165 (3628347) 3628347..3628649 - 303 WP_261175519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5O77_RS18170 (3628782) 3628782..3629576 + 795 WP_000365177.1 TraB/GumN family protein -
N5O77_RS18175 (3629780) 3629780..3630259 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N5O77_RS18180 (3630283) 3630283..3631083 + 801 WP_000439796.1 hypothetical protein -
N5O77_RS18185 (3631080) 3631080..3631583 + 504 WP_000667000.1 hypothetical protein -
N5O77_RS18190 (3631621) 3631621..3633273 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3620659..3631583 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11825.40 Da        Isoelectric Point: 10.0576

>T258329 WP_261175519.1 NZ_CP104533:c3628649-3628347 [Escherichia coli]
MAQNKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT258329 WP_000806442.1 NZ_CP104533:c3628311-3627970 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References