Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3783242..3783936 Replicon chromosome
Accession NZ_CP104526
Organism Escherichia coli strain E8

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag N5O73_RS18515 Protein ID WP_001263493.1
Coordinates 3783242..3783640 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N5O73_RS18520 Protein ID WP_000554757.1
Coordinates 3783643..3783936 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3778902) 3778902..3778982 - 81 NuclAT_8 - -
- (3778902) 3778902..3778982 - 81 NuclAT_8 - -
- (3778902) 3778902..3778982 - 81 NuclAT_8 - -
- (3778902) 3778902..3778982 - 81 NuclAT_8 - -
N5O73_RS18485 (3778242) 3778242..3779486 - 1245 WP_000189541.1 esterase FrsA -
N5O73_RS18490 (3779578) 3779578..3780036 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N5O73_RS18495 (3780297) 3780297..3781754 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
N5O73_RS18500 (3781811) 3781811..3782332 - 522 Protein_3623 peptide chain release factor H -
N5O73_RS18505 (3782331) 3782331..3782534 - 204 Protein_3624 RtcB family protein -
N5O73_RS18510 (3782780) 3782780..3783232 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
N5O73_RS18515 (3783242) 3783242..3783640 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5O73_RS18520 (3783643) 3783643..3783936 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5O73_RS18525 (3783988) 3783988..3785043 - 1056 WP_001226164.1 DNA polymerase IV -
N5O73_RS18530 (3785114) 3785114..3785899 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
N5O73_RS18535 (3785871) 3785871..3787583 + 1713 Protein_3630 flagellar biosynthesis protein FlhA -
N5O73_RS18540 (3787799) 3787799..3788296 - 498 WP_000006256.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T258309 WP_001263493.1 NZ_CP104526:c3783640-3783242 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258309 WP_000554757.1 NZ_CP104526:c3783936-3783643 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References