Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3063851..3064578 Replicon chromosome
Accession NZ_CP104526
Organism Escherichia coli strain E8

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag N5O73_RS14930 Protein ID WP_000558568.1
Coordinates 3064267..3064578 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag N5O73_RS14925 Protein ID WP_000990392.1
Coordinates 3063851..3064270 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O73_RS14900 (3058957) 3058957..3059331 + 375 Protein_2920 transposase -
N5O73_RS14905 (3059677) 3059677..3060279 - 603 WP_048266682.1 DUF6448 family protein -
N5O73_RS14910 (3060508) 3060508..3061710 + 1203 WP_219401014.1 AraC family transcriptional regulator -
N5O73_RS14915 (3061707) 3061707..3062687 + 981 WP_261245577.1 IS5-like element ISKpn26 family transposase -
N5O73_RS14920 (3062810) 3062810..3063814 + 1005 WP_223878993.1 phage tail protein -
N5O73_RS14925 (3063851) 3063851..3064270 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
N5O73_RS14930 (3064267) 3064267..3064578 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
N5O73_RS14935 (3064789) 3064789..3065169 + 381 Protein_2927 gluconate permease -
N5O73_RS14940 (3065219) 3065219..3065914 - 696 WP_000370412.1 lactate utilization protein C -
N5O73_RS14945 (3065907) 3065907..3067334 - 1428 WP_059239965.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
N5O73_RS14950 (3067345) 3067345..3068064 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T258307 WP_000558568.1 NZ_CP104526:c3064578-3064267 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT258307 WP_000990392.1 NZ_CP104526:c3064270-3063851 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References