Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5148935..5149537 Replicon chromosome
Accession NZ_CP104523
Organism Escherichia coli strain E7

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N5O80_RS25090 Protein ID WP_000897305.1
Coordinates 5148935..5149246 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5O80_RS25095 Protein ID WP_000356397.1
Coordinates 5149247..5149537 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O80_RS25065 (5144849) 5144849..5145448 + 600 WP_001315111.1 glucose-1-phosphatase -
N5O80_RS25070 (5145442) 5145442..5146314 + 873 WP_000920762.1 virulence factor BrkB family protein -
N5O80_RS25075 (5146311) 5146311..5146748 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N5O80_RS25080 (5146793) 5146793..5147734 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N5O80_RS25085 (5147798) 5147798..5148706 - 909 WP_001318161.1 alpha/beta hydrolase -
N5O80_RS25090 (5148935) 5148935..5149246 + 312 WP_000897305.1 hypothetical protein Toxin
N5O80_RS25095 (5149247) 5149247..5149537 + 291 WP_000356397.1 NadS family protein Antitoxin
N5O80_RS25100 (5150142) 5150142..5150360 + 219 WP_001295676.1 CopG family transcriptional regulator -
N5O80_RS25105 (5150575) 5150575..5151504 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N5O80_RS25110 (5151501) 5151501..5152136 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N5O80_RS25115 (5152133) 5152133..5153035 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258290 WP_000897305.1 NZ_CP104523:5148935-5149246 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258290 WP_000356397.1 NZ_CP104523:5149247-5149537 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References