Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4244936..4245735 Replicon chromosome
Accession NZ_CP104523
Organism Escherichia coli strain E7

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag N5O80_RS20675 Protein ID WP_000347251.1
Coordinates 4245271..4245735 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag N5O80_RS20670 Protein ID WP_001296435.1
Coordinates 4244936..4245271 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O80_RS20655 (4240721) 4240721..4241491 - 771 WP_261306712.1 2-dehydro-3-deoxyglucarate aldolase -
N5O80_RS20660 (4241507) 4241507..4242841 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
N5O80_RS20665 (4243216) 4243216..4244787 + 1572 WP_001273941.1 galactarate dehydratase -
N5O80_RS20670 (4244936) 4244936..4245271 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5O80_RS20675 (4245271) 4245271..4245735 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5O80_RS20680 (4245790) 4245790..4246599 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N5O80_RS20685 (4246848) 4246848..4248128 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5O80_RS20690 (4248151) 4248151..4248624 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5O80_RS20695 (4248635) 4248635..4249414 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5O80_RS20700 (4249404) 4249404..4250282 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N5O80_RS20705 (4250300) 4250300..4250734 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4237240..4245735 8495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T258283 WP_000347251.1 NZ_CP104523:4245271-4245735 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT258283 WP_001296435.1 NZ_CP104523:4244936-4245271 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References