Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1233497..1234176 Replicon chromosome
Accession NZ_CP104523
Organism Escherichia coli strain E7

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag N5O80_RS05830 Protein ID WP_000057523.1
Coordinates 1233497..1233799 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N5O80_RS05835 Protein ID WP_000806442.1
Coordinates 1233835..1234176 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O80_RS05810 (1228828) 1228828..1230048 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
N5O80_RS05815 (1230266) 1230266..1231850 + 1585 Protein_1133 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
N5O80_RS05820 (1231887) 1231887..1232366 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N5O80_RS05825 (1232570) 1232570..1233364 - 795 WP_000365161.1 TraB/GumN family protein -
N5O80_RS05830 (1233497) 1233497..1233799 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5O80_RS05835 (1233835) 1233835..1234176 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N5O80_RS05840 (1234234) 1234234..1236738 - 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
N5O80_RS05845 (1237001) 1237001..1237933 + 933 WP_261306735.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1233497..1243580 10083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T258267 WP_000057523.1 NZ_CP104523:1233497-1233799 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT258267 WP_000806442.1 NZ_CP104523:1233835-1234176 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References