Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4932486..4933088 Replicon chromosome
Accession NZ_CP104521
Organism Escherichia coli strain E5

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N5O78_RS23870 Protein ID WP_000897305.1
Coordinates 4932486..4932797 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5O78_RS23875 Protein ID WP_000356397.1
Coordinates 4932798..4933088 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O78_RS23845 (4928400) 4928400..4928999 + 600 WP_001315111.1 glucose-1-phosphatase -
N5O78_RS23850 (4928993) 4928993..4929865 + 873 WP_000920762.1 virulence factor BrkB family protein -
N5O78_RS23855 (4929862) 4929862..4930299 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N5O78_RS23860 (4930344) 4930344..4931285 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N5O78_RS23865 (4931349) 4931349..4932257 - 909 WP_001318161.1 alpha/beta hydrolase -
N5O78_RS23870 (4932486) 4932486..4932797 + 312 WP_000897305.1 hypothetical protein Toxin
N5O78_RS23875 (4932798) 4932798..4933088 + 291 WP_000356397.1 NadS family protein Antitoxin
N5O78_RS23880 (4933693) 4933693..4933911 + 219 WP_001295676.1 CopG family transcriptional regulator -
N5O78_RS23885 (4934126) 4934126..4935055 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N5O78_RS23890 (4935052) 4935052..4935687 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N5O78_RS23895 (4935684) 4935684..4936586 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258260 WP_000897305.1 NZ_CP104521:4932486-4932797 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258260 WP_000356397.1 NZ_CP104521:4932798-4933088 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References