Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4075919..4076718 Replicon chromosome
Accession NZ_CP104521
Organism Escherichia coli strain E5

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag N5O78_RS19725 Protein ID WP_000347251.1
Coordinates 4076254..4076718 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag N5O78_RS19720 Protein ID WP_001296435.1
Coordinates 4075919..4076254 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O78_RS19705 (4071704) 4071704..4072474 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
N5O78_RS19710 (4072490) 4072490..4073824 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
N5O78_RS19715 (4074199) 4074199..4075770 + 1572 WP_094122420.1 galactarate dehydratase -
N5O78_RS19720 (4075919) 4075919..4076254 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5O78_RS19725 (4076254) 4076254..4076718 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5O78_RS19730 (4076773) 4076773..4077582 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N5O78_RS19735 (4077831) 4077831..4079111 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5O78_RS19740 (4079134) 4079134..4079607 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5O78_RS19745 (4079618) 4079618..4080397 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5O78_RS19750 (4080387) 4080387..4081265 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N5O78_RS19755 (4081283) 4081283..4081717 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T258254 WP_000347251.1 NZ_CP104521:4076254-4076718 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT258254 WP_001296435.1 NZ_CP104521:4075919-4076254 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References