Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1172971..1173650 Replicon chromosome
Accession NZ_CP104521
Organism Escherichia coli strain E5

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag N5O78_RS05530 Protein ID WP_000057523.1
Coordinates 1172971..1173273 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N5O78_RS05535 Protein ID WP_000806442.1
Coordinates 1173309..1173650 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O78_RS05510 (1168243) 1168243..1169463 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
N5O78_RS05515 (1169681) 1169681..1171324 + 1644 WP_094122321.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
N5O78_RS05520 (1171361) 1171361..1171840 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N5O78_RS05525 (1172044) 1172044..1172838 - 795 WP_000365161.1 TraB/GumN family protein -
N5O78_RS05530 (1172971) 1172971..1173273 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5O78_RS05535 (1173309) 1173309..1173650 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N5O78_RS05540 (1173708) 1173708..1176212 - 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
N5O78_RS05545 (1176475) 1176475..1177407 + 933 WP_000883024.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1172971..1183054 10083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T258241 WP_000057523.1 NZ_CP104521:1172971-1173273 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT258241 WP_000806442.1 NZ_CP104521:1173309-1173650 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References