Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2063710..2064251 Replicon chromosome
Accession NZ_CP104516
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag N5O79_RS10585 Protein ID WP_000615981.1
Coordinates 2063973..2064251 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N5O79_RS10580 Protein ID WP_000729704.1
Coordinates 2063710..2063970 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O79_RS10560 (2059393) 2059393..2060178 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
N5O79_RS10565 (2060150) 2060150..2061862 + 1713 Protein_2077 flagellar biosynthesis protein FlhA -
N5O79_RS10570 (2062078) 2062078..2062575 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
N5O79_RS10575 (2062751) 2062751..2063524 - 774 WP_001353765.1 C40 family peptidase -
N5O79_RS10580 (2063710) 2063710..2063970 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5O79_RS10585 (2063973) 2063973..2064251 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5O79_RS10590 (2064407) 2064407..2065147 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5O79_RS10595 (2065118) 2065118..2065885 - 768 WP_000333379.1 class II glutamine amidotransferase -
N5O79_RS10600 (2066091) 2066091..2066669 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T258223 WP_000615981.1 NZ_CP104516:2063973-2064251 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT258223 WP_000729704.1 NZ_CP104516:2063710-2063970 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References