Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2057521..2058215 Replicon chromosome
Accession NZ_CP104516
Organism Escherichia coli strain E4

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag N5O79_RS10545 Protein ID WP_001263493.1
Coordinates 2057521..2057919 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N5O79_RS10550 Protein ID WP_000554757.1
Coordinates 2057922..2058215 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2053181) 2053181..2053261 - 81 NuclAT_13 - -
- (2053181) 2053181..2053261 - 81 NuclAT_13 - -
- (2053181) 2053181..2053261 - 81 NuclAT_13 - -
- (2053181) 2053181..2053261 - 81 NuclAT_13 - -
N5O79_RS10515 (2052521) 2052521..2053765 - 1245 WP_000189541.1 esterase FrsA -
N5O79_RS10520 (2053857) 2053857..2054315 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N5O79_RS10525 (2054576) 2054576..2056033 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
N5O79_RS10530 (2056090) 2056090..2056611 - 522 Protein_2070 peptide chain release factor H -
N5O79_RS10535 (2056610) 2056610..2056813 - 204 Protein_2071 RtcB family protein -
N5O79_RS10540 (2057059) 2057059..2057511 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
N5O79_RS10545 (2057521) 2057521..2057919 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5O79_RS10550 (2057922) 2057922..2058215 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5O79_RS10555 (2058267) 2058267..2059322 - 1056 WP_001226164.1 DNA polymerase IV -
N5O79_RS10560 (2059393) 2059393..2060178 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
N5O79_RS10565 (2060150) 2060150..2061862 + 1713 Protein_2077 flagellar biosynthesis protein FlhA -
N5O79_RS10570 (2062078) 2062078..2062575 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T258222 WP_001263493.1 NZ_CP104516:c2057919-2057521 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp


Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT258222 WP_000554757.1 NZ_CP104516:c2058215-2057922 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References