Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4261170..4261897 Replicon chromosome
Accession NZ_CP104509
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N5O74_RS21565 Protein ID WP_000550189.1
Coordinates 4261583..4261897 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N5O74_RS21560 Protein ID WP_000560266.1
Coordinates 4261170..4261586 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O74_RS21550 (4256330) 4256330..4258681 + 2352 WP_000695487.1 alpha-glucosidase -
N5O74_RS21555 (4259107) 4259107..4261125 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N5O74_RS21560 (4261170) 4261170..4261586 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N5O74_RS21565 (4261583) 4261583..4261897 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N5O74_RS21570 (4262181) 4262181..4263317 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N5O74_RS21575 (4263402) 4263402..4263905 + 504 WP_001333820.1 M48 family metallopeptidase -
N5O74_RS21580 (4263982) 4263982..4264674 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
N5O74_RS21585 (4264753) 4264753..4265739 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T258203 WP_000550189.1 NZ_CP104509:c4261897-4261583 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT258203 WP_000560266.1 NZ_CP104509:c4261586-4261170 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References