Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 950633..951174 Replicon chromosome
Accession NZ_CP104509
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag N5O74_RS04810 Protein ID WP_000615983.1
Coordinates 950633..950911 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag N5O74_RS04815 Protein ID WP_000729703.1
Coordinates 950914..951174 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O74_RS04795 (948215) 948215..948793 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N5O74_RS04800 (948999) 948999..949766 + 768 WP_000333380.1 class II glutamine amidotransferase -
N5O74_RS04805 (949737) 949737..950477 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5O74_RS04810 (950633) 950633..950911 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5O74_RS04815 (950914) 950914..951174 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5O74_RS04820 (951384) 951384..952133 + 750 WP_000056849.1 C40 family peptidase -
N5O74_RS04825 (952309) 952309..952806 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N5O74_RS04830 (953030) 953030..954742 - 1713 Protein_935 flagellar biosynthesis protein FlhA -
N5O74_RS04835 (954714) 954714..955499 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 929163..958874 29711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T258187 WP_000615983.1 NZ_CP104509:c950911-950633 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT258187 WP_000729703.1 NZ_CP104509:c951174-950914 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References