Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4243134..4243828 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag N5O81_RS20525 Protein ID WP_001263501.1
Coordinates 4243430..4243828 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag N5O81_RS20520 Protein ID WP_000554755.1
Coordinates 4243134..4243427 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS20495 (4238473) 4238473..4238970 + 498 WP_000006241.1 REP-associated tyrosine transposase RayT -
N5O81_RS20500 (4238965) 4238965..4239459 - 495 WP_139174989.1 hypothetical protein -
N5O81_RS20505 (4239487) 4239487..4241199 - 1713 Protein_4007 flagellar biosynthesis protein FlhA -
N5O81_RS20510 (4241171) 4241171..4241956 + 786 WP_000207543.1 putative lateral flagellar export/assembly protein LafU -
N5O81_RS20515 (4242027) 4242027..4243082 + 1056 WP_001226170.1 DNA polymerase IV -
N5O81_RS20520 (4243134) 4243134..4243427 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N5O81_RS20525 (4243430) 4243430..4243828 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N5O81_RS20530 (4243838) 4243838..4244290 + 453 WP_001059897.1 GNAT family N-acetyltransferase -
N5O81_RS20535 (4244480) 4244480..4245619 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
N5O81_RS20540 (4245616) 4245616..4246230 + 615 WP_000602123.1 peptide chain release factor H -
N5O81_RS20545 (4246287) 4246287..4247744 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
N5O81_RS20550 (4248005) 4248005..4248463 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T258175 WP_001263501.1 NZ_CP104503:4243430-4243828 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT258175 WP_000554755.1 NZ_CP104503:4243134-4243427 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References