Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 587052..587605 Replicon chromosome
Accession NZ_CP104503
Organism Escherichia coli strain E1

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag N5O81_RS03005 Protein ID WP_000605680.1
Coordinates 587336..587605 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag N5O81_RS03000 Protein ID WP_000781370.1
Coordinates 587052..587336 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O81_RS02985 (582233) 582233..585280 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
N5O81_RS02990 (585293) 585293..586177 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
N5O81_RS02995 (586170) 586170..586823 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
N5O81_RS03000 (587052) 587052..587336 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
N5O81_RS03005 (587336) 587336..587605 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5O81_RS03010 (587791) 587791..588801 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
N5O81_RS03015 (588935) 588935..590632 - 1698 WP_000433464.1 malate dehydrogenase -
N5O81_RS03020 (590789) 590789..590926 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
N5O81_RS03025 (591028) 591028..591243 - 216 WP_000495766.1 biofilm-dependent modulation protein -
N5O81_RS03030 (591588) 591588..592019 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T258158 WP_000605680.1 NZ_CP104503:c587605-587336 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT258158 WP_000781370.1 NZ_CP104503:c587336-587052 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References