Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4003224..4003765 Replicon chromosome
Accession NZ_CP104500
Organism Escherichia coli strain E9

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag N5O75_RS20005 Protein ID WP_000615976.1
Coordinates 4003487..4003765 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N5O75_RS20000 Protein ID WP_000729704.1
Coordinates 4003224..4003484 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O75_RS19975 (3999007) 3999007..3999792 - 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -
N5O75_RS19980 (3999764) 3999764..4001476 + 1713 Protein_3914 flagellar biosynthesis protein FlhA -
N5O75_RS19985 (4001581) 4001581..4001859 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
N5O75_RS19990 (4001852) 4001852..4002208 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
N5O75_RS19995 (4002265) 4002265..4003038 - 774 WP_000543895.1 C40 family peptidase -
N5O75_RS20000 (4003224) 4003224..4003484 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N5O75_RS20005 (4003487) 4003487..4003765 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N5O75_RS20010 (4003921) 4003921..4004661 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N5O75_RS20015 (4004632) 4004632..4005399 - 768 WP_000333380.1 class II glutamine amidotransferase -
N5O75_RS20020 (4005605) 4005605..4006183 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T258147 WP_000615976.1 NZ_CP104500:4003487-4003765 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT258147 WP_000729704.1 NZ_CP104500:4003224-4003484 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References