Detailed information of TA system TA258017

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87408..87828
• Replicon: plasmid pUC4224_1
• Accession: NZ_CP104473
• Organism: Escherichia coli strain UC4224

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: N4S17_RS25040
• Protein ID: WP_223200913.1
• Coordinates: 87408..87530 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	87634..87828 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N4S17_RS25010 (83214)	83214..83597	-	384	WP_021521311.1	conjugal transfer relaxosome DNA-binding protein TraM	-
N4S17_RS25015 (84051)	84051..84530	+	480	WP_249821647.1	transglycosylase SLT domain-containing protein	-
N4S17_RS25020 (84826)	84826..85647	-	822	WP_028985438.1	DUF932 domain-containing protein	-
N4S17_RS25025 (85765)	85765..86052	-	288	WP_000107526.1	hypothetical protein	-
N4S17_RS25030 (86208)	86208..86510	-	303	WP_001272236.1	hypothetical protein	-
N4S17_RS25035 (86810)	86810..87107	+	298	Protein_91	hypothetical protein	-
N4S17_RS25040 (87408)	87408..87530	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
N4S17_RS25045 (87475)	87475..87627	-	153	Protein_93	DUF5431 family protein	-
- (87634)	87634..87828	-	195	NuclAT_0	-	Antitoxin
- (87634)	87634..87828	-	195	NuclAT_0	-	Antitoxin
- (87634)	87634..87828	-	195	NuclAT_0	-	Antitoxin
- (87634)	87634..87828	-	195	NuclAT_0	-	Antitoxin
N4S17_RS25050 (87777)	87777..88091	-	315	Protein_94	theronine dehydrogenase	-
N4S17_RS25055 (88088)	88088..88807	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
N4S17_RS25060 (88804)	88804..89238	-	435	WP_000845927.1	conjugation system SOS inhibitor PsiB	-
N4S17_RS25065 (89293)	89293..91251	-	1959	WP_050864290.1	ParB/RepB/Spo0J family partition protein	-
N4S17_RS25070 (91316)	91316..91549	-	234	WP_000005988.1	DUF905 family protein	-
N4S17_RS25075 (91607)	91607..92128	-	522	WP_000290783.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..111840	111840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T258017 WP_223200913.1 NZ_CP104473:c87530-87408 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 195 bp

>AT258017 NZ_CP104473:c87828-87634 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin