Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3858030..3858571 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag N4S17_RS18565 Protein ID WP_000615976.1
Coordinates 3858030..3858308 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N4S17_RS18570 Protein ID WP_000729704.1
Coordinates 3858311..3858571 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS18550 (3855612) 3855612..3856190 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N4S17_RS18555 (3856396) 3856396..3857163 + 768 WP_000333380.1 class II glutamine amidotransferase -
N4S17_RS18560 (3857134) 3857134..3857874 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N4S17_RS18565 (3858030) 3858030..3858308 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N4S17_RS18570 (3858311) 3858311..3858571 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N4S17_RS18575 (3858757) 3858757..3859530 + 774 WP_000543899.1 C40 family peptidase -
N4S17_RS18580 (3859587) 3859587..3859943 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
N4S17_RS18585 (3859936) 3859936..3860214 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
N4S17_RS18590 (3860319) 3860319..3862031 - 1713 Protein_3626 flagellar biosynthesis protein FlhA -
N4S17_RS18595 (3862003) 3862003..3862788 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T258007 WP_000615976.1 NZ_CP104472:c3858308-3858030 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT258007 WP_000729704.1 NZ_CP104472:c3858571-3858311 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References